Saracens scrum-half Ben Spencer has been added to England's squad after Dan Robson was ruled out due to illness.
Tommy Seymour, Blair Kinghorn and Stuart Hogg will miss Scotland's final Six Nations match, while France will also be without two players.
Maro Itoje will be unable to aid England's bid to win the Six Nations after injury ruled him out of the Scotland game.
Having suffered defeat to Bristol last time out, Gloucester responded in emphatic fashion with a resounding away win over Harlequins.
With Italy losing their last 21 Six Nations outings, Eddie Jones feels organisers should discuss changes to the tournament's structure.
England scored eight tries in a rout, but Italy head coach Conor O'Shea said: "I wouldn't get too carried away with the scoreline."
Head coach Eddie Jones says England must look after themselves on what will be a tense final weekend of the Six Nations season.
England responded to their Six Nations loss versus Wales with an emphatic defeat Italy, setting up an intriguing final weekend.
Gregor Townsend was left to reflect on a missed opportunity after Scotland failed to derail Wales' quest for a Grand Slam.
Lewis Moody says England will be wounded from a defeat to Wales that he says should never have happened.
The fourth round of fixtures in the 2019 Six Nations sees Wales visit Scotland, England take on Italy and Ireland host France.
Italy captain Sergio Parisse will make his 68th Six Nations appearance when the Azzurri face England on Saturday.
Eddie Jones has made five changes to the England team to take on Italy, Joe Cokanasiga and Ben Te'o among the players coming in.
Maro Itoje limped out of England training and Charlie Ewels has been called up as cover for the lock.
British and Irish Lions duo Maro Itoje and Jonathan Joseph, as well as former skipper Chris Robshaw, have been called up by England.
Bill Beaumont will chair a meeting in Dublin this month to discuss plans for a new annual 12-team global competition.